VIP 10mg (10 vials/kit): Neuropeptide & Immunomodulatory Reagent for Vasodilation & Anti-Inflammatory Research

VIP (Vasoactive Intestinal Peptide) is a highly conserved 28-amino-acid endogenous neuropeptide belonging to the secretin/glucagon/growth hormone-releasing factor ($\text{GHRF}$) superfamily. Characterized by the sequence $\text{His-Ser-Asp-Ala-Val-Phe-Thr-Asp-Asn-Tyr-Thr-Arg-Leu-Arg-Lys-Gln-Met-Ala-Val-Lys-Lys-Tyr-Leu-Asn-Ser-Ile-Leu-Asn-NH}_2$, VIP acts as a potent neurotransmitter and cytokine-like immunomodulator. In biological systems, VIP binds with high affinity to two class B G-protein-coupled receptors: $\text{VPAC}_1$ and $\text{VPAC}_2$. Sourcing VIP 10mg (10 vials/kit) provides neuroscientists, immunologists, and cardiovascular researchers with a high-purity analytical reagent engineered to evaluate smooth muscle relaxation, pulmonary vasodilation, neuroprotection, microglial suppression, and regulatory T-cell ($\text{T}_{\text{reg}}$) homeostasis in vitro and in vivo.

At the cellular level, binding of VIP to $\text{VPAC}_1$ or $\text{VPAC}_2$ receptors activates heterotrimeric $\text{G}_s$ proteins, stimulating adenylyl cyclase ($\text{AC}$) to rapidly elevate intracellular cyclic adenosine monophosphate ($\text{cAMP}$) levels. Elevated $\text{cAMP}$ triggers protein kinase A ($\text{PKA}$) phosphorylation, which suppresses nuclear factor-$\kappa\text{B}$ ($\text{NF-}\kappa\text{B}$) transactivation while promoting cAMP response element-binding protein ($\text{CREB}$) phosphorylation:

This dual signaling axis inhibits the production of pro-inflammatory cytokines ($\text{TNF-}\alpha$, $\text{IL-6}$, $\text{IL-12}$, $\text{iNOS}$) in activated macrophages and microglia, while upregulating anti-inflammatory mediators such as interleukin-10 ($\text{IL-10}$). In vascular and airway smooth muscle cells, VIP-induced $\text{PKA}$ activation leads to intracellular calcium sequestration and hyperpolarization, driving rapid nitric oxide-independent vasodilation and bronchodilation.

The VIP 10mg (10 vials/kit) configuration yields a total kit payload of 100mg active peptide material (10mg per vial across ten individual USP Type I borosilicate glass vials). Freeze-dried under sterile vacuum conditions, each vial contains a uniform, highly stable lyophilized cake. This multi-vial format allows research personnel to reconstitute single working units for active bioassays while keeping remaining stock preserved in deep-freeze conditions to protect against thermal degradation and secondary oxidation.

Receptor Selectivity, Anti-Inflammatory Signaling, and Technical Profile

The primary scientific value of VIP 10mg (10 vials/kit) stems from its dual $\text{VPAC}_1/\text{VPAC}_2$ receptor activation, potent vasorelaxant properties, and multi-pathway immunomodulatory cascades:

• Dual VPAC1/VPAC2 Receptor Agonism: High-affinity binding to class B GPCRs ($\text{VPAC}_1$ and $\text{VPAC}_2$) drives downstream $\text{cAMP/PKA}$ secondary messenger signaling across neurological and immune tissues.

• Potent Vasodilation & Smooth Muscle Relaxation: Induces direct, endothelium-independent relaxation of vascular and bronchial smooth muscle, lowering arterial and pulmonary pressures in cardiovascular models.

• Microglial & Neuroprotective Modulation: Crosses neuro-vascular interfaces in experimental models to inhibit microglial activation, counteracting neuroinflammatory damage and apoptosis in neurodegenerative assays.

• Immune Tolerance & Treg Induction: Modulates dendritic cell maturation to promote antigen-specific regulatory T-cell ($\text{T}_{\text{reg}}$) differentiation while suppressing Th1/Th17 inflammatory responses.

• Ultra-High Chemical Purity ($\ge$99.0%): Manufactured via automated Solid-Phase Peptide Synthesis ($\text{SPPS}$) and purified using preparative High-Performance Liquid Chromatography ($\text{HPLC}$) to ensure complete removal of truncation sequences and synthesis artifacts.

Reconstitution Guidelines, Laboratory Storage Standards, and Quality Protocols

Achieving reproducible, publication-grade results when working with VIP 10mg (10 vials/kit) requires strict adherence to standardized laboratory preparation and climate-controlled storage protocols.

Perform all reconstitution procedures inside a certified Class II Biosafety Cabinet:

1. Temperature Balancing: Allow the frozen VIP vial to adjust to ambient room temperature prior to solvent introduction to eliminate moisture condensation inside the glass container.

2. Septum Sanitization: Remove the protective plastic flip-cap and thoroughly wipe the synthetic rubber stopper with a fresh 70% isopropyl alcohol pad. Allow the surface to dry completely.

3. Slow Diluent Addition: Using a sterile syringe equipped with a fine-gauge needle, draw the required volume of Sterile Water, Normal Saline (0.9% NaCl), or Phosphate-Buffered Saline (PBS). Direct the liquid stream against the inner glass wall so it trickles slowly over the lyophilized cake.

4. Gentle Hydration: Allow the diluent to fully hydrate the powder cake for 2 minutes. Gently roll the vial between your palms or swirl in smooth circular motions until clear. Never vortex or shake violently, as high-shear agitation can disrupt peptide secondary structures and induce protein foaming.

5. Micro-Aliquot Strategy: For multi-week testing protocols, divide reconstituted liquid into sterile, single-use microcentrifuge tubes under aseptic conditions to prevent atmospheric oxidation and eliminate repeated freeze-thaw cycles.

Batch Quality Documentation & Traceability: Every production lot of VIP 10mg (10 vials/kit) undergoes third-party analytical testing. High-Performance Liquid Chromatography (HPLC) chromatograms confirm component purity exceeding 99.0%, while Electrospray Ionization Mass Spectrometry (ESI-MS) confirms precise theoretical molecular mass. Each shipment includes a batch-specific Certificate of Analysis (COA) documenting chemical purity, residual solvent analysis, heavy metal screens, and bacterial endotoxin levels (<0.05 EU/mg) to satisfy institutional compliance requirements.

Strict Regulatory & Research Compliance Statement: VIP 10mg (10 vials/kit) is produced, packaged, and distributed exclusively for in vitro laboratory research, academic experimentation, chemical synthesis, and preclinical research. VIP 10mg (10 vials/kit) is strictly not intended, formulated, or approved for human or animal consumption, therapeutic medical treatment, clinical diagnostic work, or cosmetic applications. All handling must be conducted by certified research personnel operating within equipped laboratory facilities in compliance with OSHA workplace safety regulations and institutional biosafety protocols.